Extended Data Fig. 10: Nuclear MiTF as read-out of KITLG-KIT signaling is detectable in MCs localizing along KITLG-expressing stromal cells in the periarteriolar space.
From: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
(a) Immunofluorescence staining analysis of nuclear microphthalmia-associated transcription factor (MiTF) signal in dermal MCs of ear skin whole mount tissue. MC surfaces (purple) were generated based on avidin staining (red). Only MiTF signals inside MC surfaces are displayed as single color (green) or as heatmap. In contrast to MCs in the interstitial spaces, many of the periarteriolar MCs show strong nuclear MiTF signals, suggesting upregulated KITLG–KIT signaling in MCs localizing in the periarteriolar space. Representative image of n = 3 independent experiments. (b–d) GFP-expressing fibroblasts that reside along dermal arterioles in KitlGFP/+ mice were phenotypically characterized by immunofluorescence stainings against desmin (b), chondroitin sulfate proteoglycan (Ng2) (c) and vimentin (d). Representative images of n = 4 independent experiments (b–d). Scale bars: 50 µm (a), and 5 µm (b–d).
