Fig. 6: Phenotypes of the T cell subclusters with the highest positive and negative correlation to SARS-CoV-2 spike peptide-induced IFNγ production.
a, Heatmap showing the median intensity of normalized marker expression (range: 0–1) for canonical T cell subsets and identified T cell subclusters (n = 347). b, Differential marker expression by the specific T cell subclusters compared to the canonical T cell subsets that positively and negatively correlated with SARS-CoV-2 spike peptide-induced IFNγ responses, filtered for markers with at least 0.2 differential expression. Color and bubble size indicate the differential expression values compared to the canonical cell type (n = 347). c, Participants were classified as negative (<1.03, the detection threshold) or positive (>1.03) for IFNγ response and mean antibody levels and spike-binding mBC marker expression levels were compared between negative and positive participants. Boxes bound the IQR divided by the median, and Tukey-style whiskers extend to a maximum of 1.5× IQR beyond the box. Dots are participant data points (n = 255). d, The count and percentage of participants classified as negative or positive responders, grouped by vaccine regimen at T1 and T3 (n = 328 at T1, n = 255 at T3).
