Extended Data Fig. 3: Genes highly expressed in liMacs.
From: Lactation-associated macrophages exist in murine mammary tissue and human milk
a-e, scRNA-seq analysis of myeloid cells (CD11b+ and/or CD11c+) of lactating mammary glands derived from WT mice at 7 dpp. a, Heatmap of cluster-defining genes. Related to Fig. 2a. b, Dot plot showing expression of selected ‘Folr2 signature’ genes22 in liMacs, F4/80hi macrophages (Mac) and F4/80lo macrophages. Dot size represents percentage of cells in a cluster expressing each gene; dot color reflects expression level (as indicated in the legend). c, Heatmap showing expression of macrophage and DC signature genes67,68 in liMacs, F4/80lo macrophages, F4/80hi macrophages, cDC2s and cDC1s. d, Dot plot showing expression of selected ‘Cadm1/Trem2 signature’ genes22 in liMacs, F4/80hi macrophages and F4/80lo macrophages. Dot size represents percentage of cells in a cluster expressing each gene; dot color reflects expression level (as indicated in the legend). e, Enrichment of GO terms according to the gene expression pattern in liMacs, F4/80hi macrophages and F4/80lo macrophages of lactating mammary glands. f, Immunofluorescence image of lactating mammary glands from Cx3cr1GFP/+ dams (day 8 pp) showing DAPI (blue), SMA (white), Cx3cr1-GFP (green) and Dectin-1 (red) (left) and single stainings for Cx3cr1-GFP and Dectin-1 (right). Images are representative of n = 2 mice. Scale bar: 100 µm.
