Extended Data Fig. 2: tDC originate from BM progenitors.

a Gating strategy used for the analysis of skin-draining lymph node (LN) and lung DC subsets excluded. Lineage contains CD3⁺/CD19⁺/NK1.1⁺/Ly6G⁺ cells. b Bar graphs (mean + SD) showing the contribution of donor cells to LN and lung DC populations (gated as in (a)) in mixed BMC generated by transplanting 50% CD45.1 WT and 50% CD45.2 TCF4^cKO BM, normalized to NK cells (n = 6 mice in 2 experiments). Statistics determined by two-way ANOVA with Dunnett’s multiple comparison test. c Bar graph (mean + SD) showing the percentage of splenic DC (gated as in Fig. 1f) labeled with the lineage tracing CD300c^TdT (n = 4 mice in 3 experiments). d Adoptive cell transfer of 30,000 BM (left) or splenic (right) CX3CR1^EGFP CD45.1 pDC into CD45.2 WT non-irradiated congenic mice. Recipient mice were euthanized at different time points (2–8-days) and the number of recovered cells (gated on CD45.1⁺ and CX3CR1⁺) calculated per 1000 cells transferred (mean + SD). N = 3/time point except n = 2 for splenic pDC at 8-days. N represents number of mice and experiments. e CyTOF analysis of blood and spleen DC, analyzed by UMAP. Cells were gated as CD3^-CD19^-NK1.1^-Ly6G^-. Populations were delineated manually (top) and colored by marker expression (bottom)(n = 2 mice in 2 experiments). f Spleen, blood and BM of hCD2^EYFP mice were analyzed for the presence of EYFP⁺ cells, that were further separated in Ly6D⁺ pDC and CX3CR1⁺ tDC. Lineage includes CD3⁺/CD19⁺/NK1.1⁺/Ly6G⁺ cells. One representative of 3 experiments.

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