Extended Data Fig. 6: tDC have lower turnover rate than pre-DC2 and required IRF4.
a Mice were inoculated i.p. with 1 mg of BrdU on day 0 and then fed continuously for 14 days with 0.8 mg/mL of BrdU in drinking water. After 14 days, BrDU was removed. Mice were euthanized at different time points post-BrdU removal, spleen cell suspension prepared and stained for BrdU and the identification of DC subsets as described in Figs. 1f and 5e. N ≥ 2/mice per time point in 2 independent experiments. b Expression of Ki-67 in each splenic DC population gated as described in Fig. 1f and 5e, analyzed by flow cytometry. One representative of 3 experiments. c BM cells from IRF4cKO or IRF4control mice were cultured with FLT3L as described in Fig. 2c. At day 6, tDC were sorted and re-cultured in complete media and 1% FLT3L for 1 and 2 days. Bar graphs (mean + SD) show the percentage of each DC recovered. N = 3 biologically independent samples in 3 experiment. d Bar graphs (mean + SD) of the total number of splenic DC (gated a Fig. 1f and 4f) in hCD2EYFP CX3CR1DTR mice inoculated with DT every second day (n = 3 mice) or left untreated (n = 3 mice), and analyzed at day 10. Statistics determined by unpaired two-tailed t-test. e As in (d), but shown is the number of other immune cells.
