Extended Data Fig. 4: Age-related changes in A2/M158+CD8+ TCRαβ repertoire.
a, Z-score for intra- versus inter-donor distance, larger Z means intra-donor distances are smaller than inter-donor distances, that is greater heterogenticity across donors. b, TRAV and TRBV clonotype pairing for individual donors within each age group illustrated by circos plots. Left arch segment colors indicate TRAV usage, right outer arch colors depict TRBV usage. Connecting lines indicated TRAV-TRBV gene pairing and are colored based on their TRAV usage and segmented based on their CRD3α and CDR3β sequence, the thickness is proportional to the number of TCR clones with the respective pair. The number of sequences considered for each circos plot is shown at the right bottom. c, Frequency of high (TCRs detected ≥2 within a single individual) and low (TCRs detected once with a single individual) prevalent public (shared) clonotypes across individuals of age groups. Dark red represents high prevalent public TCR (TRAV27, TRAJ42, CDR3α GAGGGSQGNLIF, TRBV19, TRBV2-7, CDR3β CASSIRSSYEQYF), whereas the light red are clonotypes expressing the full public TCRβ chain (TRBV19, TRBV2-7, CDR3β CASSIRSSYEQYF) but TCRα chain could not be identified. Numbers in the squares represent the number of donors in which the specific high versus low prevalent clonotype was identified. d, Frequency of high prevalent (TCRs detected ≥2 within a single individual) private (not shared) clonotypes across individuals of age groups. Statistical analysis was performed using a two-sided Kruskal-Wallis with Dunn’s correction for multiple tests. Exact significant p-values are indicated above the graphs.
