Fig. 3: Single-cell transcriptomics shows evolutionary trajectory across human life.

a, PAGA analysis of single cells (n = 793) identified five clusters (left) and their relative connectivity (middle) and colored based on the four UMAP clusters (right). b, PAGA analysis colored by age (left), phenotype (right). Single-cell phenotypes were obtained via index sorting. c, PAGA analysis colored by public TCR features (left), TRBV27 expression (middle) and clone size (right). Bar charts represent respective age, phenotype and TCR features distributions in each PAGA cluster. d, Trajectory derived from scRNA-seq data with colored pseudotime values (large panel). Loess curves represent the changes along the trajectories of age groups (top left), phenotype based on protein expression (top right), TCR features (middle) and the dot-plot shows expression values of selected genes measured via scaled mean expression along the pseudotime size of the dots corresponds to the percentage of cells expressing the gene in the given pseudotime state (bottom). Colored lines are a Loess fit, with error bands shaded in matching colors representing the 95% CI (d, bottom left). N, newborn; C, children; A, adult; OA, older adult.