Extended Data Fig. 4: Analysis of the BCR repertoire.

a, The stack column graphic shows the percentages of clones with each isotype for each single sample of ASCs from different tissues as indicated in the plot. Undetermined, indicates the clones which we were unable to link to an isotype. b-c, The boxplots show the value distribution of CDR3 lengths for clones with each isotype (b) and the value distribution of mutation rates for clones in ASCs from different tissues by isotype (c). A dot represents one single clone that has a outlier value. The central lines indicate the group median values, the top and bottom lines indicate the 75th and 25th percentiles, respectively, the whiskers represent minima and maxima within the 1.5× the interquartile range. d-e, Quantification of the Ighv repertoire diversity. d, The Gini-Simpson index was calculated for all TrPC populations. A value near 0 would indicate a monoclonal sample, while a value of 1 would be infinite diversity. e, Ighv gene usage percentage accumulation curves for clones in ASCs from different tissues by isotype. Each single line represents clones from a single tissue of the same isotype. The first dot of each line represents the percentage of the most abundant Ighv gene, the second dot represents the sum of the percentages of the first two most abundant Ighv genes, and so forth. A curve with a higher slope indicates a lower diversity.