Extended Data Fig. 3: GATA3 controls ST2⁺ VAT Treg cell differentiation.

a, FACS plots show ST2 and GATA3 expression in VAT Treg cells of male and female mice. b, GATA3 expression in CD4⁺ Foxp3⁻and Foxp3⁺ cells of Gata3^fl/flFoxp3^Cre and control mice. c, Graphs show frequencies of total (left) and activated (right) splenic Treg cells of Gata3^fl/flFoxp3^Cre (left n = 4 females, n = 6 males; right n = 8 females, n = 13 males) and control (left n = 8 females, n = 11 males; right n = 8 females, n = 7 males) mice. d, Treg cell frequencies and numbers in small intestine lamina propria (n = 13 control, n = 6 Gata3^fl/flFoxp3^Cre) and lung (n = 10 per genotype). e, Numbers of ST2⁺ and CXCR3⁺ VAT Treg cells in Gata3^fl/flFoxp3^Cre (n = 6 each sex) and control (n = 8 females, n = 7 males) mice. f, Frequencies (control n = 12 each sex; Gata3^fl/flFoxp3^Cre n = 10 females, n = 11 males) and numbers (control n = 12 females, n = 11 males; Gata3^fl/flFoxp3^Cre n = 10 each sex) of VAT Treg cells. g, Numbers of TNF⁺ (control n = 9 females, n = 15 males; Gata3^fl/flFoxp3^Cre n = 4 females, n = 12 males), IL-5⁺ (control n = 8 females, n = 10 males; Gata3^fl/flFoxp3^Cre n = 5 females, n = 7 males) and IFN-γ⁺ (control n = 8 females, n = 10 male; Gata3^fl/flFoxp3^Cre n = 5 females, n = 7 males) cells. h, Body (control n = 13 females, n = 15 males; Gata3^fl/flFoxp3^Cre n = 11 females, n = 10 males) and VAT (control n = 8 females, n = 14 males; Gata3^fl/flFoxp3^Cre n = 10 females, n = 15 males) weights of male and female Gata3^fl/flFoxp3^Cre and control mice. i, Body composition of male and female Gata3^fl/flFoxp3^Cre and control mice (control n = 4 females, n = 5 male; Gata3^fl/flFoxp3^Cre n = 5 females, n = 4 males). Data representative (a, b) or pooled (c-i) from at least two independent experiments. Symbols represent individual mice, error bars indicate standard deviation, horizontal lines are means. Statistical analyses performed using two-way ANOVA or unpaired, two-tailed Student’s t-test (d).