Extended Data Fig. 6: ST2+ and CXCR3+ VAT Treg cells have distinct functions.
From: Two regulatory T cell populations in the visceral adipose tissue shape systemic metabolism
a, Graphs show numbers of CD8 (control n = 22 females, n = 32 males; Ppargfl/flFoxp3Cre n = 17 each sex; Tbx21fl/flFoxp3Cre n = 11 each sex) and CD4 T cells (control n = 22 females, n = 32 males; Ppargfl/flFoxp3Cre n = 17 each sex; Tbx21fl/flFoxp3Cre n = 8 each sex) and TNF (control n = 21 females, n = 26 males; Ppargfl/flFoxp3Cre n = 9 females, n = 5 males; Tbx21fl/flFoxp3Cre n = 4 females, n = 10 males) or IFNγ (control n = 21 females, n = 28 males; Ppargfl/flFoxp3Cre n = 9 females, n = 5 males; Tbx21fl/flFoxp3Cre n = 8 females, n = 12 males) producing TCRβ+ cells in the VAT of male and female Tbx21fl/flFoxp3Cre, Ppargfl/flFoxp3Cre and control mice. b, VAT Treg cell quantification in male and female Tbx21fl/flFoxp3Cre, Ppargfl/flFoxp3Cre and control mice on HFD (control n = 10 females, n = 10 males; Tbx21fl/flFoxp3Cre n = 9 females, n = 8 males; Ppargfl/flFoxp3Cre n = 9 females, n = 14 males). c, d, Insulin tolerance was assessed in Ppargfl/flFoxp3Cre mice (c) and Tbx21fl/flFoxp3Cre mice (d) under conditions of normal diet (ND) and high fat-diet (HFD) feeding. ND on top and HFD bottom, females on left, males on right. Symbols represent individual mice (a, b) or mean of 3−5 mice (c, d). Data are pooled from 2−3 independent experiments. Error bars indicate the standard deviation (a, b) or s.e.m. (c, d). Statistical analyses were performed using two-way ANOVA (a, b) or unpaired, two-tailed Student’s t-test (c, d).
