Extended Data Fig. 8: ST2 and CXCR3 + VAT Treg cells regulate distinct inflammatory pathways.

a, b, Distribution of dendritic cell and macrophage populations in the VAT of male Tbx21^fl/flFoxp3^Cre (n = 5), Pparg^fl/flFoxp3^Cre (n = 8) and control mice under normal diet (ND, n = 4) and high fat diet (HFD, n = 5) conditions. (a) Macrophage frequencies (left) and numbers on right (b) Dendritic cell frequencies (left) and numbers on right. c, Representative flow cytometry plot showing expression of IL-5 by ST2⁺ Treg cells in male mice. d-f, (d) Representative FACS plots showing Cxcl9 and Cxcl10 reporter expression in male and female REX3 reporter mice. (e) Frequencies (left) and numbers (right) of Cxcl9⁺ cells in the VAT CD45⁺ compartment of male (n = 4) and female (n = 6) REX3 mice. (f) Frequencies (left) and numbers (right) of Cxcl10⁺ cells in the VAT CD45⁺ compartment of male (n = 4) and female (n = 6) REX3 mice. g, Gating strategy for macrophage and dendritic cells in the VAT. h, Graph showing frequency of Cxcl10 (left) and Cxcl9 (right) expressing macrophages and dendritic cells in male (n = 4) and female (n = 6) REX3 mice. Symbols represent individual mice; horizontal lines indicate means. Data are pooled from at least two independent experiments (a, b, e, f, h); FACS plots are representative (c, d, g). Error bars indicate the standard deviation. Statistical analyses were performed using two-way ANOVA (a, b, h) or unpaired, two-tailed Student’s t-test (e, f).