Extended Data Fig. 5: HSC and Mk signatures of HSPCs replenished by single transplanted Vwf-tdTomato⁻ Multi-HSCs or Vwf-tdTomato⁺ P-HSCs.

a, MolO HSC (top) and MkP (bottom) AUC signature scores for HSPCs replenished by Vwf-tdTomato⁺ P-HSCs or Vwf-tdTomato⁻ Multi-HSCs along pseudotime. Dots represent individual cells and lines represent LOESS curves (local regression to fit a smooth curve through scatterplot) of AUC signature for the HSC subtype with 95% confidence interval (CI). b, Low output, high output, Mk-bias and multilineage AUC signature scores for HSPCs replenished by Vwf-tdTomato⁺ P-HSCs or Vwf-tdTomato⁻ Multi-HSCs along pseudotime. Dots represent individual cells and lines represent LOESS curves (local regression to fit a smooth curve through scatterplot) of AUC signature for the HSC subtype with 95% CI. c, d, AUC scores for stem cell associated signatures in HSCs (c) and for lineage-restricted progenitor signatures in HSCs and MkPs (d) replenished by Vwf-tdTomato⁺ P-HSCs (n = 1047 HSC and 119 MkPs from 7 mice) or Vwf-tdTomato⁻ Multi-HSCs (n = 97 HSCs and 177 MkPs from 8 mice), based on published gene signatures (see Methods). Boxes show first and third quartiles of the normalized expression values, where the line within each box indicates the median, whiskers indicate the largest value within the ±1.5*IQR, and dots indicate outlier cells. * P < 0.05, ** P < 0.01, **** P < 0.0001, ns P > 0.05 (non-significant) by two-sided Wilcoxon test. P values: MolO = 3.29 × 10⁻¹³, Low output = 9.84 × 10⁻¹¹, High output = 4.88 × 10⁻⁶, Mk bias = 8.58 × 10⁻¹³, Multilineage = 0.0022, HSC1 cluster = 4.88 × 10⁻⁶, Serial engrafter = 1.37 × 10⁻¹¹, Stem-score = 0.15, SuMO = 4.48 × 10⁻⁵, RACFP-DIM = 0.15, dHSCvsaHSC = 0.97, HSC = 2.54 × 10⁻¹³, HSC-MkP = 6.06 × 10⁻²¹, MkP-MkP = 0.72, HSC-CFUE = 4.08 × 10⁻²⁴, MkP-CFUE = 2.65 × 10⁻¹¹, HSC-GMP = 1.90 × 10⁻²⁴, MkP-GMP = 0.72, HSC-CLP = 0.02, MkP-CLP = 0.72.