Extended Data Fig. 9: Validation of anti-NP2 antibody. | Nature Immunology

Extended Data Fig. 9: Validation of anti-NP2 antibody.

From: Macrophages and nociceptor neurons form a sentinel unit around fenestrated capillaries to defend the synovium from circulating immune challenge

Extended Data Fig. 9

(a) Immunocytochemical analysis of HA-tagged NP1, NP2 and NPR (full-length) expressed in HEK293 cells. Newly raised anti-NP2 antibody showed selective signals for NP2. (b) Specific immunostaining of endogenous NP2 in the visual cortex after the light stimulation. Anti-NP2 antibody detected the increase of NP2 protein expression in all layers of visual cortex 4 hours after light exposure compared with 0 hr exposure control. Bars, 50 μm.

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