Supplementary Figure 4: Quantification for modular combinations of AAV–Cpf1-mediated efficient multiplex knockin and knockout in human primary CD4+ T cells.
From: One-step generation of modular CAR-T cells with AAV–Cpf1

a, Quantification of dTomato-knockin frequency at TRAC target sites shown in a dot-plot graph (uninfected n = 2 cell culture replicates; AAV vector n = 3 infection replicates; PDCD1KO;dTomato-TRACKI n = 3 infection replicates). AAV vector sample was infected only with AAV6 carrying the same construct, and did not receive electroporation of Cpf1 thereafter. Unpaired two-sided t test was used to assess significance. Vector versus TRAC-KIKO, **P < 0.01. Data are shown as mean ± s.e.m., plus individual data points on the graph. b, Quantifications of GFP-knockin frequency at PDCD1 target site shown in a dot-plot graph (cell culture or infection replicates, n = 6). Unpaired t test was used to assess significance. Vector versus PDCD1-KIKO, ***P < 0.001. Data are shown as mean ± s.e.m., plus individual data points on the graph. c, Quantification of percentages of GFP+ and dTomato+ cells shown in a dot-plot graph with one vector system (infection replicates, AAV vector n = 3; dTomato-TRACKI;GFP-PDCD1KI n = 4). Unpaired two-sided t test was used to assess significance. Vector versus dTomato-TRACKI;GFP-PDCD1KI, ***P < 0.001 for all knockin comparisons (GFP+, dTomato+ and GFP+dTomato+). Data are shown as mean ± s.e.m., plus individual data points on the graph. d, Quantification of percentages of GFP+ and dTomato+ cells shown in a dot-plot graph with two-vector system (infection replicates, n = 3). Unpaired two-sided t test was used to assess significance. Vector versus dual targeting, ***P < 0.001 for all knockin comparisons (GFP+, dTomato+ and GFP+dTomato+). Data are shown as mean ± s.e.m., plus individual data points on the graph.