Supplementary Figure 2: Evaluation of in vivo library diversity in the absence of mutagenesis.
From: In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens
a, Experimental design to evaluate the suitability of the in vivo transplant model for high-throughput genetic interrogation. To model neutral selection in the absence of mutagenesis, KPD cells were infected with a lentiviral library containing random 8-mer barcodes, for a theoretical total of 48 = 65,536 unique barcodes. Twelve days after injection into mice, genomic DNA was extracted from the nodules for barcode sequencing and assessment of library diversity. b, Bar plot detailing the percentage of all possible 8-mers (n = 65,536) that were recovered in each sample (cell pool, n = 1 infection replicate; nu/nu mice, n = 2 mice; Rag–/– mice, n = 4 mice). c, Tukey box plots (IQR boxes with 1.5 × IQR whiskers and all outliers shown individually) of the abundances of all possible 8-mers (n = 65,536) in each sample (cell pool, n = 1 infection replicate; nu/nu mice, n = 2 mice; Rag–/– mice, n = 4 mice).
