Supplementary Figure 3: Segmentation of dendritic cells.
From: Robust and automated detection of subcellular morphological motifs in 3D microscopy images
(a) A MIP across 4 µm of a deconvolved image of a dendritic cell expressing LifeAct-GFP. This image was segmented by processing the deconvolved image into three images that accentuate different features: (b) a renormalized deconvolved image, (c) an image that emphasizes the interior of the cell, and (d) an image that emphasizes planar features, such as lamellipodia. These three images were combined into (e) a composite image. (f) Finally, a mesh was generated from an isosurface of the composite image.
