Extended Data Fig. 10: DI-SPA quantification of proteins from mitochondria subcellular fractions.
From: Quantitative shotgun proteome analysis by direct infusion
a, Overlap of 37 mitochondrial proteins quantified: 149 from the purified mitochondria experiment and 56 from the whole cell experiment. b, Heatmap showing trend of general decrease in the 149 proteins annotated mitochondrial from DI-SPA analysis of the purified mitochondria. Light is the signal from the experimental condition and heavy is the signal from the SILAC standard protein. *Benjamini-Hochberg adjusted p-value <0.05, exact corrected p-values: PHB=0.023, CISY=0.023, THIL=0.036, CH10 = 0.036. n = 3 independent biological replicates of mitochondria preparations from 293 T cells from one independent experiment. p-values are from a two-tailed T-test assuming equal variance, and corrected p-value is from Benjamini-Hochberg multiple hypothesis testing correction. Source Data is available as Supplementary Table 6.
