Extended Data Fig. 2: Examples of infusion data traces.
From: Quantitative shotgun proteome analysis by direct infusion
Tryptic peptides from the MCF7 proteome (1 mg/mL) were infused as described for DI-SPA analysis, but precursor ions (MS1) were measured. a, Description of general flowgram parameters over time. b, MS1 trace of the no FAIMS experiment (top) and extracted ion chromatograms of various randomly chosen multiply charged m/z values (±10ppm) show a consistent pattern of elution for all masses. This suggests that peptides are not retained or separated in our setup. c, Comparison of the signal from without FAIMS versus FAIMS using each CV setting from −30 V to −80 V. d,.Example flow-gram from DI-SPA-PRM-MS of 100 fmol/μL angiotensin II showing the typical smooth trace of mass- and FAIMS-selected peptide precursor.
