Extended Data Fig. 2: Tests of resolution and full FOV imaging.
From: Miniature three-photon microscopy maximized for scattered fluorescence collection
(a-d) Left, x-y and y-z images, averaged from 50 frames; right, normalized and averaged lateral and axial profile of beads (n = 5 in a-b, n = 12 in c-d), yielding a full width at half-maximum in silicon oil immersion at the center of FOV (a), in water immersion at the center of the FOV (b), at the 4 corner positions of a 220×220 μm2 FOV (c), and at the 4 corner positions of a 400×400 μm2 FOV (d). Data are shown as mean ± s.e.m. (e) 50-frame averaged x-y image of neurons labeled with GCaMP6s in posterior parietal cortex (PPC) (L6, depth of 590 μm) with a 400 × 400 μm2 FOV at a frame rate of 8.35 Hz and 200 × 200 pixels per frame. The experiment was independently repeated n = 4 times. (f) Time courses of calcium activities of the indexed neurons expressing GCaMP6s in (e). Traces were 3-frame moving averaged. The optical power after the objective is 4.2 mW.
