Extended Data Fig. 3: The design of the m3PM system.
From: Miniature three-photon microscopy maximized for scattered fluorescence collection
(a) The microscope system’s design with inset shows a profile of the m3PM headpiece. HWP, half-wave plate; PBS, polarizing beam splitter; HC-1300, hollow-core fiber for 1300 nm transmission; SFB, supple fiber bundle; DM, dichroic mirror; MEMS, micro-electro-mechanical systems; TL, tube lens; SL, scan lens; SIMO, simplified infinite miniature objective. (b) The x-y plot of the laser beam after Fiber-01. Inner, electron micrograph cross-section and an image of 1300 nm laser light delivered by Fiber-01 after collimation by a doublet. (c) The laser pulse temporal profile at the exit of the 1.5 m Fiber-01, after slight adjustment of the built-in dispersion compensation of the laser. (d) The x-y plot of the laser beam after Fiber-02. Inner, electron micrograph cross-section and an image of 1300 nm laser light delivered by Fiber-02 after collimation by an aspheric lens. (e) The laser pulse temporal profile at the exit of the 1.6 m Fiber-02 without dispersion compensation.
