Extended Data Fig. 6: Triple-plane imaging of somatic activity in the dorsal hippocampus CA1.
From: Miniature three-photon microscopy maximized for scattered fluorescence collection
(a) Schematic of the m3PM headpiece with the 2-gram z-scanning module (ZSM). The ZSM consists of a fast electrically tunable lens (ETL) and a pair of relay lenses. We integrated the ETL, the relay lens (RL) pair and the MEMS to form a 4 F (conjugated) system, in which the equal pupil plane of the ETL was conjugated to the surface of MEMS. The ZSM can be easily removed from the headpiece without affecting the performance of the headpiece itself. HC-1300, hollow-core optical fiber for 1300 nm transmission; MEMS, microelectromechanical systems; SL, scan lens; TL, tube lens; DM, dichroic mirror; SIMO, simplified infinite miniature objective; SFB, supple fiber bundle. (b) a snapshot of the m3PM headpiece. (c) Triple-plane imaging of somatic activity in the hippocampus CA1 at 885, 905 and 927 μm depth with a cycle rate of 4.4 Hz. We acquired a frame on each plane and looped for 3500 times. Top, 50-frame averaged x-y images. Bottom, time courses calcium activities of the indexed neurons expressing GCaMP6s in x-y images. Traces were 3-frame moving averaged. The optical power after the objective is 25.6 mW. The experiment was independently repeated n = 4 times.
