Extended Data Fig. 10: RNA subcellular dynamics in neonatal primary skin culture.

a, RNA reads (cDNA amplicons) per cell for each pulse-chase timepoint in primary human skin cell culture. b-c, stacked bar plots summarizing the fraction of reads in each subcellular region of all cells at each timepoint when combining all cells (b) and separating into cardiomyocytes and fibroblasts (c). Vertical lines indicate s.d. d, PCA representation showing the gene clustering of 256 genes using all eight estimated parameters across cell cycle. e, UMAP visualization of single cells showing the averaged expression level of each gene in cluster 1-3 across all timepoints. Color indicates the level of averaged RNA expression. f, UMAP visualization of single cells based on the expression level of PMEL and MITF (melanocyte markers), COL1A1 and COL6A1 (fibroblast markers), and KRT14 and KRT5 (keratinocyte markers). Color indicates the level of RNA expression. g-h, Heatmap depicting the pairwise correlation of 256 genes in the primary skin cells by TEMPOmap-measured single-cell RNA covariation when combining four chase timepoints (0, 2, 4, 6 hrs chase), where the color indicates the value of Pearson correlation (g). Group 1-3 are highlighted for highly correlated gene modules (left) and zoomed in (h).