Extended Data Fig. 4: PhAST rescues calcium signaling but not behavior in the eat-4(ky5) mutation.
From: Neural engineering with photons as synaptic transmitters
(a,b) Schematic of the experimental circuit manipulation. Nodes are colored according to the LUT in (b), gray dotted edges indicate genetically perturbed synaptic connections in the eat-4(ky5) mutation. (b) Image plot and average jRGECO1a fluorescence after nose touch in the microfluidic device for AVA (khaki, blue) and AIB (olive, violet) in eat-4(ky5) animals expressing the light pathway in absence of the cofactors. Shaded area indicates SD around the mean (black trace). The vertical bar indicates the duration of the mechanical stress. (c,d) Schematic of the circuit with TeNL expression in ASH (blue) and light-restored edges shown with blue arrows. (d) Image plot and average jRGECO1a fluorescence for AVA (khaki, blue) and AIB (olive, violet) for the same animals as in (b) but in presence of both cofactors. Shaded area indicates SD around the mean (black trace). (e) Behavioral response of eat-4(ky5) animals expressing the light pathway in presence and absence of all cofactors. (f) Summary of nose touch in eat-4(ky5) animals. Each blue dot corresponds to the average from ten touch tests per animal, N=30 animals in total. Black dot and vertical bars indicate median ± 95% confidence interval (CI). Numbers on gray brackets indicate the two-sided p-value derived from a Wald test. The floating axis indicates the bootstrapped distribution of the paired median difference (PMD) between HIKarazine single treated animals and double-treated animals. Red point indicates median, vertical bar 95%CI. Overlap of the CI with zero indicates low effect size and likely statistically insignificant distributions. Inset shows the log-odds ratio of finding a treated animal responding compared to the untreated mutant control.
