Extended Data Fig. 8: Red-shifted Luciferase/ChRmine pair is not sufficient to replace endogenous neurotransmitters.
From: Neural engineering with photons as synaptic transmitters
(a) i) Representative fluorescence micrograph (N=8) of the individual transgenes used to express CaMBI in ASH and ChRmine:SL2:jRGeCO1a in AIB and AVA. ii) Bioluminescence of the CaMBI in ASH. Scale bars = 40 μm. (b) Quantification of the nose touch response of animals expressing ChRmine in AVA and AIB in presence an absence of all cofactors. Each red dot corresponds to the average from ten touch tests per animal, One representative experiment displayed of a total of three (N=30 animals). Only for display purposes, a scatter of 10% was applied to each datapoint to avoid overlap. Black circle indicates median, vertical bar indicates 95% confidence interval on the median. Floating axis indicates the paired median difference (PMD), derived from bootstrapping 100 independent distributions from the experimental data set. Red point indicates median, red vertical bar indicates 95%CI. Overlap of the CI with zero indicates low effect size and likely statistically insignificant distributions. Numbers on gray brackets indicate the two-sided p-value derived from a Wald test.
