Extended Data Fig. 1: Nose touch avoidance behavior in wild-type and eat-4 vesicular glutamate transporter mutant.
From: Neural engineering with photons as synaptic transmitters
(a) Schematic of the behavioral assay. The yes/no response of a single animal is recorded as it navigates into a user-controlled obstacle (eyebrow hair). (b, c) Wiring of the nociceptive avoidance circuit with the representative result of an experiment of 10 trials conducted on 10 animals in (b) wild-type and (c) eat-4(ky5) mutants. Circuit nodes colored according to their neurotransmitter (yellow=glutamate; red=acteylcholine; gray=silent). Dashed arrow indicates broken connection in the eat-4(ky5) mutation. Grid plots show the yes/no response, color-coded according to its outcome, of individual animals as they navigated into the eyebrow hair. (d) Summary of touch tests of both genotypes. Each blue dot corresponds to the average from ten touch tests per animal, N = number of tested animals in total. A jitter was applied to visually separate individual datapoints only for plotting purposes. Only positive datapoints are shown. Floating axis on the right shows the bootstrapped distribution of their paired median difference (right axis). Filled circle indicates median, vertical bar indicates 95%CI on the median. Two-sided p-value derived from a Wald test.
