Extended Data Fig. 9: The gRNA-dependent off-target levels of CBEs at the in-silico predicted off-target sites. | Nature Methods

Extended Data Fig. 9: The gRNA-dependent off-target levels of CBEs at the in-silico predicted off-target sites.

From: Development of miniature base editors using engineered IscB nickase

Extended Data Fig. 9

The left and right panel is off-target effects of miCBE and SpG-CBE targeting EMX1, VEGFA and PCSK9 genes respectively. The underlined sequences represent the on-target or predicted off-target sites. The mismatches were labeled in red and lower case. Each point represents the editing efficiency of independent biological replicates by deep sequencing analysis. Data are presented as means ± s.d., n = 3 independent biological replicates.

Source data

Back to article page