Fig. 3: Connectivity reconstruction in live hippocampus.
From: Dense 4D nanoscale reconstruction of living brain tissue
a, Orthogonal planes from an isotropically super-resolved LIONESS volume in xy and xz directions in neuropil of the DG in organotypic hippocampal slice culture from a Prox1-cre::Ai95 mouse. White arrowheads at image edges indicate position of corresponding orthogonal planes. The image is representative of n > 20 repetitions. Scale bar, 2 µm. LIONESS images are maximum intensity projections spanning 150 nm. b, 3D reconstructions of example cellular structures extracted from a. c, 3D reconstruction of a spiny dendrite from a, showing various spine shapes (left), its embedding in dense neuropil (middle) and the 29 axons making a total of 39 putative synaptic connections at 38 spines (right). The scale cube refers to the center of the renderings. d, Distribution of spine lengths for the dendrite in c. e, Spine ___location (horizontal bars) and relative spine lengths (white portion of bars) along the dendrite (gold) with 3D renderings of example putative synaptic connections. Scale bars, 1 µm, referring to the center of the spine of interest. The overall width of the golden bar corresponds to the longest observed spine (3.96 µm). Proofreading of automated segmentation and 3D visualization and analysis as in b–e was applied to one dataset.
