Extended Data Fig. 7: Publicly available human scRNA-seq PBMC dataset from the Fluent Biosciences PIPseq platform60. | Nature Methods

Extended Data Fig. 7: Publicly available human scRNA-seq PBMC dataset from the Fluent Biosciences PIPseq platform60.

From: Unsupervised removal of systematic background noise from droplet-based single-cell experiments using CellBender

Extended Data Fig. 7

Droplets are generated by vigorous vortexing, and thus we expect more ambient RNA than a microfluidics experiment. The UMAP shows the expected cell types, in addition to some probable doublets. The two dotplots show expression of immune cell marker genes before and after CellBender. Many genes show significant cleanup. UMAP plots on the right show rather marked cleanup of a few genes after CellBender.

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