Extended Data Fig. 4: Comparison between single-layer and multi-layer SIM reconstruction. | Nature Methods

Extended Data Fig. 4: Comparison between single-layer and multi-layer SIM reconstruction.

From: Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform

Extended Data Fig. 4

a, Nuclear pore complex shot by OMX. b, Actin filament in U2OS osteosarcoma cells from fairSIM. Quantitative resolution calibration of WF, HiFi-SIM, and Open-3DSIM, including results of c, Argolight, and d, sparse fluorescent beads. And the fitted profile on z-axis of e, Argolight, and f, sparse fluorescent beads to quantify the half-height full-size and resolution based on 10 randomly selected points. Scale bar: 2 μm. Scale on z-axis: a, 17 layers, b, 8 layers, c, 33 layers, d, 8 layers, 0.125 μm per layer.

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