Extended Data Fig. 10: Cocaine treatment of MISCOs.
a, Representative GCAMP recordings of 130-day-old MISCOs with Syn-GCAMP expression either in the cortical or striatal tissue in control (left), chronic (middle) and withdrawal (right) condition. b, Representative GCAMP-recording of a MISCO with Syn-GCAMP in striatal tissue before (control) and after TTX application (0.5 µM), visualized as cumulative neuronal activity in a 6.5 min recording and displayed as ΔF/F. c, Calcium event duration of VM neurons in control, chronic and withdrawal conditions. 876|2058|1068 individual calcium events of 7-17 organoids. All Padj < 0.0001. d, Analysis of calcium event frequency of VM (n = 100|278|115) neurons in control, chronic and withdrawal conditions. Padj = 0.0341|0.002|< 0.0001. Data shown as mean ± SD. Samples were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test (c,d). e-f, Volcano plots of differentially expressed genes in VM tissue in chronic versus control (e) and withdrawal versus control (f). P-value was calculated using a Benjamin-Hochberg corrected Wald test using DESeq2. g-i, GSEA analysis of three GO terms associated with neural circuit formation (dendrite development, axon development and synapse organization) in forebrain chronic vs control (top) and forebrain withdrawal versus control (bottom).
