Fig. 3: Brightness of StayGold variants and reference green-emitting FPs in live cells.
From: StayGold variants for molecular fusion and membrane-targeting applications
a, Cotranslation of green-emitting FP with mCherry using the bicistronic coexpression system. Transfection was performed with pCSII-EF/mCherry-T2A-green-emitting FP. b, Cellular brightness 48 h after transfection. The green fluorescence was corrected for the mCherry fluorescence and spectral throughput (Supplementary Fig. 7) and normalized to that of StayGold (Supplementary Table 2). Transfection was repeated four times; the mean values are shown by gray bars and are reported in Table 1 (cellular brightness). c, Fluorescence development after transfection. Side-by-side comparison of six green-emitting FPs for their chromophore maturation using an automated time-lapse imaging system that accommodates a six-well plate. The green fluorescence was corrected for the mCherry fluorescence (t = 48 h) (Supplementary Note 8) and spectral throughput (Supplementary Fig. 8). Data points are shown as mean ± s.e.m. (n = 3 independent experiments). Curves are colored as follows. QC2-6 FIQ (mSG) and QC2-6(PT) (mSG2), green; SG, dark green; EGFP, dark blue; mNeonGreen, red; mGreenLantern, magenta. Inset shows curves during the early stage.
