Extended Data Fig. 5: Evaluations of FDR (mdFDR) and power in identifying DA taxa in (a) a random intercept model, and (b) a random coefficients model.
From: Multigroup analysis of compositions of microbiomes with covariate adjustments and repeated measures
Synthetic datasets were generated using the PLN model18 based on the mean and covariance estimated from the QMP dataset23. The X-axis shows the sample size per group, and the Y-axis shows the FDR (mdFDR) or power. Each panel title designates the proportion of true DA taxa. The depicted metrics represent mean values ± standard errors (indicated by error bars) derived from 100 simulation runs for each setting. The outcomes accentuate that, when integrated with the SS filter, ANCOM-BC2 effectively moderates FDR (mdFDR) while retaining power parallel to its performance without the SS filter. In the absence of the SS filter, ANCOM-BC2 surpasses LinDA and LMM-CLR in maintaining consistently low FDR and equivalent power.
