Extended Data Fig. 2: Pharmacological profiles of GRABDA sensors measured in cultured cells.
From: Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
a, Titration curves of indicated sensors for the response to DA or NE in HEK293T cells. n = 3 cells with 400–500 cells per well, mean±s.e.m. b, The normalized ΔF/F0 in sensor-expressing HEK293T cells in response to the indicated compounds. Antagonists were applied at 10 μM, others at 1 μM. n = 4 wells for gDA3m and gDA3h, n = 3 wells for others, mean±s.e.m. One-way Anova, post hoc Dunnett’s test: gDA3m, p = 0.0002, 8.9 × 10−10 between DA and SFK, or DA+Etic, p = 1.3 × 10−13 between DA and others; gDA3h, p = 0.0020, 4.3 × 10−9 between DA and SFK, or DA+Etic, p = 1.3 × 10−13 between DA and others; rDA2m, p = 3.3 × 10−14 between DA and others; rDA2h, p = 0.6059, 0.9530 between DA and DA + SCH, or DA+Etic, p = 3.3 × 10−14 between DA and others; rDA3m, p = 0.9182, 0010 between DA and DA+Etic, or SKF, p = 3.3 × 10−14 between DA and others; rDA3h, p = 0.0724, 0.8723 between DA and DA+Etic, or Quin, p = 3.3 × 10−14 between DA and others. c, The normalized ΔF/F0 in rDA2m-expressing HEK293T cells in response to indicated DA agonists. Bromocriptine (Bro), Rotigotine (RTG), D2R/D1R agonists; Ropinirole (RPR), Quin, D2R-specific agonists; Fenodopam (FD), SKF, D1R-specific agonist. All chemicals were bath-applied in 100 μM. n = 3 wells, mean±s.e.m. One-way Anova, post hoc Dunnett’s: p = 0.1074 between DA and Bro, p = 8.0 × 10−12 between DA and others. d, Titration curves of indicated dopamine receptor antagonists. The fluorescence intensity in the presence with 10 μM DA was set as F0 and the relative fluorescence changes under indicated compound concentration were plotted. n = 3 wells with 400–500 cells per well, mean±s.e.m. e, f, Pharmacological specificity (left) and titration curves of indicated sensors for the response to DA or NE (right) in cultured neurons. Left, antagonists at 10 μM, others at 1 μM. n = 3 wells, mean±s.e.m. One-way Anova, post hoc Dunnett’s test: rDA2h, p = 0.9998, 0.1458 between DA and DA + SCH, or DA+Etic, p = 1.5 × 10−7 between DA and others; rDA3h, p = 0.9591, 0.1309 between DA and DA+Etic, or SKF, p = 4.0 × 10−9 between DA and others.
