Extended Data Fig. 3: Induced calcium fluxes in CAR T cells.
From: Multimodal probing of T-cell recognition with hexapod heterostructures
a, Representative time-lapse images of the induced calcium fluxes in 1st generation CAR T cells using anti-CD3-hexapod or CD19-hexapod, respectively. Scale bar, 5 µm. The experiment was repeated independently five times with similar results. b, Calcium profiles (mean F/F0 ± SEM,) show that anti-CD3-hexapod (n = 5 independent experiments) induced stronger calcium than CD19-hexapod (n = 5 independent experiments) in 1st generation CAR T cells, indicating that TCR transduction has higher efficiency than CAR transduction. c, NALM-6 cell induced saturated calcium signals in CAR T cells. Scale bar, 5 µm. The experiment was repeated independently for six times with similar results. d, Calcium profile (mean F/F0 ± SEM) of NALM-6 cell-induced calcium (n = 6 independent experiments). NALM-6 cells serve as a positive control. e, Calcium intensity at each time point (1 frame per two seconds or n = 300 data points) of stimulated CAR T cells. Lines represent mean F/F0 ± SD. Statistical analysis was performed using one-way ANOVA test with Tukey’s multiple comparisons.
