Fig. 2: Testing BNP-Track’s performance on two 5-μm-wide regions of interest with different emitter densities based on an experimental dataset from fluorophore-labeled mRNA molecules diffusing in live U-2 OS cells onto a dual-camera microscope.
a,b, For convenience only, we show time averages of all 22 frames analyzed from cameras A (a) and B (b). The selected ROIs are boxed, and the zoomed-in images of the indicated ROIs are shown on the right. The remaining region, ROI-3, is only highlighted and is analyzed later in the text (Fig. 5). c,d, Estimated tracks within the selected ROIs from both cameras, with solid boxes indicating the corresponding ROIs after image registration. e,f, Reconstructed time courses for individual tracks from the selected ROIs. The dotted boxes in d highlight two emitter tracks only detected by camera A. g, Time course reconstruction by combining the top and middle of e. h, Time course reconstruction by combining the top and middle of f.
