Fig. 5: BNP-Track’s performance in increasingly crowded environments.

a, Time-averaged images over 22 frames from both cameras show many diffraction-limited emitters within the ROI analyzed here (ROI-3). b, BNP-Track’s tracking results after registering FOVs. c, Comparison between the BNP-Track result and the TrackMate result with a high localization quality threshold (set A) for the data from camera A. TrackMate tracks are in black; see Tinevez et al.30 for the definition of quality. d, A comparison between the BNP-Track result and the TrackMate result with a low localization quality threshold (set B) for the data from camera A. TrackMate tracks are in black.