Extended Data Fig. 4: OPLD adjustment module.
From: Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM
a, Schematic diagram of the 4Pi-cavity. The red dashed box at the bottom indicates the OPLD coarse-tuning translation submodule (CTS) while the one on top indicates the OPLD fine-tuning translation submodule (FTS). PM: knife-edge right-angle prism protective silver mirror. b, SolidWorks model of the CTS, designed to adjust the OPLD with sub-μm resolution. c, SolidWorks model of the FTS, designed to adjust the OPLD with sub-nm resolution. d, Process of coarse adjustment of the OPLD, capturing fluctuations in the intensity of bead images as the CTS stage moves to pinpoint the maximum interference ___location. The blue line indicates the average intensity profile of 20 beads from the same field of view. The magenta dashed line indicates a fitted sinusoidal envelope. e, Process of fine adjustment of the OPLD. Another scan with FTS was performed to accurately locate the maximum interference, that is zero OPLD. The blue line indicates the average intensity profile of the same 20 beads as in d. The magenta dashed line indicates a fitted sinusoidal envelope.
