Extended Data Fig. 8: Time-lapse volumetric imaging with 4Pi-SIM using conventional fluorophores.
From: Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM
a, 4Pi-SIM image (maximum intensity projection) of the actin filaments (Lifeact-mEGFP) in a live COS-7 cell. b, Time-lapse magnified views of the boxed region denoted ‘b’ in a. c, 4Pi-SIM image (maximum intensity projection) of the actin filaments (Lifeact-mScarlet-H) in a live COS-7 cell. d, Time-lapse magnified views of the boxed region denoted ‘d’ in c. e, 4Pi-SIM image (maximum intensity projection) of the ER (HaloTag-sec61β) labeled with the JFX554 dye in a live COS-7 cell. f, Time-lapse magnified views of the boxed region denoted ‘f’ in e. Scale bar: 2 μm (a-f). Representative results are shown from two to three independent experiments.
