Extended Data Fig. 9: Two-color imaging of 4Pi-SIM using dyes with different spectra in fixed cells.
From: Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM
a, 4Pi-SIM image (maximum intensity projection) of immunolabeled ER (AF488, green) and tubulin (AF568, magenta) in a fixed COS-7 cell. b, Magnified 3D rendering of the white boxed region denoted ‘b’ in a. c, Magnified x-z cross-section along the yellow dashed line in a. d, Axial intensity profiles along the white dashed line in c. a.u., arbitrary units. e, 4Pi-SIM image (maximum intensity projection) of immunolabeled mitochondria outer membrane (AF488, green) and tubulin (AF568, magenta) in a fixed U2-OS cell. f, Magnified x-z cross-section along the dashed line labeled ‘f’ in e. g, Magnified y-z cross-section along the dashed line labeled ‘g’ in e. Scale bar: 5 μm (a, e); 1 μm (b); 500 nm (c, f, g). Representative results are shown from two to three independent experiments.
