Supplementary Figure 2: RVΔGL-Cre can be used in combination with Cre-dependent AAV for intersectional targeting of projection neurons in rats. | Nature Neuroscience

Supplementary Figure 2: RVΔGL-Cre can be used in combination with Cre-dependent AAV for intersectional targeting of projection neurons in rats.

From: Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons

Supplementary Figure 2

(a) mCherry-labeled layer 6 corticothalamic cells in barrel cortex labeled via RVΔGL-Cre injected in ventral posteromedial thalamus and AAV-FLEX-mCherry injected in barrel cortex. (b) mCherry-labeled thalamocortical neurons in ventral posteromedial thalamus labeled via RVΔGL-Cre injected in barrel cortex and AAV-FLEX-mCherry injected in ventral posteromedial thalamus. (c) Axons expressing ChrimsonR-tdTomato (Klapoetke, N.C. et al. Independent optical excitation of distinct neural populations. Nat. Methods 11, 338-46 (2014)) in barrel cortex of thalamocortical cells labeled via RVΔGL-Cre injected in barrel cortex and AAV-FLEX-ChrimsonR-tdTomato injected in ventral posteromedial thalamus. (d) ChrimsonR-tdTomato-expressing axons in ventral posteromedial thalamus labeled via RVΔGL-Cre injected in ventral posteromedial thalamus and AAV-FLEX-ChrimsonR-tdTomato injected in barrel cortex. Experiments in this figure were performed four times for each condition with similar results in all cases. Scale bar: 66 μm, applies to all panels.

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