Supplementary Figure 9: Silencing of leg motor cortex neurons abolishes leg motor control in trained rats. | Nature Neuroscience

Supplementary Figure 9: Silencing of leg motor cortex neurons abolishes leg motor control in trained rats.

From: Cortico–reticulo–spinal circuit reorganization enables functional recovery after severe spinal cord contusion

Supplementary Figure 9

(Timeline) Timeline of experimental procedures in intact (n = 6) and contused rats (n = 5). (Step 1) Intact rats were evaluated during locomotion along the irregularly spaced rungs of a ladder, before and after CNO administration. The circular plots report the percent of hits, misses and slips onto the rungs of the ladder for both conditions. (Step 2) The rats received a 250 kdyn contusion. (Step 3) They followed the standard neurorehabilitation protocols as described in Supplementary Fig. 2. (Step 4) Stick diagram decomposition of leg movements during swimming. Successive leg endpoint trajectories, including velocity vector at return stroke onset, together with the oscillation of the limb and occurrence of left and right power strokes during swimming. A representative sequence is shown for intact and contused rats, both without and with CNO. Contused rats were tested without neuromodulation. ***P < 0.001. Paired Student’s t-test. (Step 5) 3D reconstruction of the cortical region with neurons expressing hM4Di, including a photograph through the coronal plane marked (1). Scale bar, 500 µm.

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