Supplementary Figure 9: Single-nucleus RNA-sequencing of occipital cortex from three females with Rett syndrome.
From: Characterization of human mosaic Rett syndrome brain tissue by single-nucleus RNA sequencing
(a) Graph clustering grouped nuclei from the three individuals based on their specific brain cell types. Nuclei clustered similarly regardless of MECP2 transcriptotype. Graph clustering was performed as described in the methods except that the significantly misregulated (FDR < 0.01, monocle2) genes in mutant excitatory neurons were omitted from the variable genes used to cluster the nuclei. (b) The number of significantly misregulated genes (FDR < 0.01, monocle2) in 953 randomly sampled excitatory neurons that have been matched to both the number and transcript depth of VIP interneurons. The mean and standard deviation of 10 iterations of excitatory neuron sampling are shown. The number of significantly misregulated genes (FDR < 0.01, monocle2) in VIP interneurons is shown as a comparison, indicating the greater number of genes detected in Fig. 2 is largely due to the number of neurons and reads detected.
