Supplementary Figure 5: Characterization of non-cell-autonomous effects in female Mecp2+/– mice.
From: Characterization of human mosaic Rett syndrome brain tissue by single-nucleus RNA sequencing
(a) Mean fold-change in gene expression of wild-type excitatory neurons from female Mecp2-/+ mice (671 cells) compared to wild-type excitatory neurons from female Mecp2+/+ control mice (sampled 671 cells) binned according to gene body DNA methylation level derived from mouse excitatory neurons (Pearson’s r = 0.04, mCA/CA, 25 gene bins, 2 gene step). (b) Mean fold-change in gene expression of wild-type excitatory neurons from female Mecp2-/+ mice compared to wild-type excitatory neurons from female Mecp2+/+ control mice (Pearson’s r = 0.02, binned according to gene length (25 gene bins, 2 gene step). The correlations in A and B were not significantly different from the respective correlations observed when comparing sets of excitatory neurons obtained by combining randomly sampled wild-type neurons from both Mecp2+/+ and Mecp2+/- mice (A, permutation test, P = 0.55; B, permutation test, P = 0.73). The lines represent mean fold-change in expression for genes binned as described; the ribbon is s.e.m. of each bin.
