Supplementary Figure 4: Electrophysiological analysis shows the neuronal property of iPSC-derived cINs in vitro.

Related to Fig. 2. The lines used are summarized in Supplementary Table 3. (a) Representative trace of action potentials induced by depolarizing current injection into cINs near threshold (30 pA, 800 ms long) and recorded in vitro in current-clamp mode (C-clamp) at approximate –70 mV. The experiment was repeated independently in 41 cells with similar results. (b) Representative traces of currents induced by voltage pulses in cINs in vitro. Membrane potential was held at –70 mV in voltage clamp mode (V-Clamp). Left, square voltage steps (800 ms) from –80 mV to –10 mV in increments of 10 mV induced both transient inward and sustained outward currents, mediated by voltage-gated Na⁺ and K⁺ channels, respectively. Right, the same trace expanded to visualize the transient inward current. The experiment was repeated independently in 31 cells with similar results. (c) Intrinsic membrane property of iPSC-derived cINs in vitro, including resting membrane potential (RMP), afterhyperpolarization (AHP), action potential threshold, and action potential half-width in cINs in the healthy control (n = 4 lines using average of 4~7 neurons per line) and schizophrenia groups (n = 4 lines using average of 3~6 neurons per line). Data are presents as mean ± SEM. Welch’s t-test (two-sided) was used for statistical analysis.