Supplementary Figure 1: Immunocytochemistry analysis of generated cINs for cIN phenotypes. | Nature Neuroscience

Supplementary Figure 1: Immunocytochemistry analysis of generated cINs for cIN phenotypes.

From: Dysregulated protocadherin-pathway activity as an intrinsic defect in induced pluripotent stem cell–derived cortical interneurons from subjects with schizophrenia

Supplementary Figure 1

Related to Fig. 1. The lines used in these experiments are summarized in Supplementary Table 3. After 8 weeks’ differentiation, cINs were stained for (a) β-Tubulin III, (b) SOX6 and (c) GAD1. (Scale bar = 100μm). Similar results were obtained upon repetition of 3 independent differentiations. (d) Cell counting analysis of cINs used in this study. Percentages of cells positive for each marker were quantified in relation to DAPI-stained total nuclei from 3 independent differentiations. 500 cells were counted for each line. There was no significant difference among different lines based on a one-way ANOVA (β-Tubulin III p = 0.0629 for healthy control and p = 0.1244 for schizophrenia; SOX6 p = 0.8148 for healthy control and p = 0.1042 for schizophrenia; GAD1 p = 0.0973 for healthy control and p = 0.3592 for schizophrenia). Data are presented as mean ±SEM.

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