Supplementary Figure 3: Controls for microglia death following demyelination in vivo.
From: Central nervous system regeneration is driven by microglia necroptosis and repopulation
a. Gating strategy for all living and dying cells excluding debris (labelled ‘cells’). Myeloid cells were detected by CD11b expression (PerCP). Detection of microglia was based on low expression of CD45 (BV605). Dying cells were detected by 7-AAD and Annexin-V (FITC). b. Flow cytometry plot of sham-lesioned mouse (taken at 7 days post-injection) showing microglia populations detected in injection site that are negative for cell death markers Annexin-V and 7-AAD. This experiment was performed with 3 mice. c. Mean number of Annexin-V+ 7-AAD+ CD11b+ CD45lo cells isolated from in vivo lesions at 3, 7, and 10 dpl ± s.e.m. *P = 0.0219, 3 vs 7 dpl (Kruskal-Wallis test). N = 3 mice per time point.
