Supplementary Figure 4: HSV-Zfp189 expression and behavior.
From: Stress resilience is promoted by a Zfp189-driven transcriptional network in prefrontal cortex
a) Injection of HSV-Zfp189 in PFC increases Zfp189 mRNA relative to HSV-GFP (t=2.718, p = 0.024, n = 10). b) Exposure to CSDS reduces OFT exploration in HSV-GFP mice (χ2(3) = 10.903, p = 0.012, n = 8-14, Mann Whitney post hoc p = 0.031), but not HSV-Zfp189 mice. However, unstressed mice show baseline differences (n = 8,11,10,14). c) Defeat reduces locomotion regardless of whether Zfp189 is overexpressed (F1,41 = 30.26, p < 0.001, n = 9,12,10,14). d) Null effects of Zfp189 overexpression in FST (χ2(3) = 0.564, p = 0.905, n = 9,11,10,14). e-g) Null effects in (h) OFT (t = 0.049, p = 0.962), (i) locomotor behavior (t = 0.077, p = 0.450), and (j) FST (t = 0.142, p = 0.888) for previously susceptible mice injected with Zfp189 in PFC (n = 12,13). *p < 0.05, **p < 0.01, ***p < 0.001. Bar graphs show mean ± SEM.
