Extended Data Fig. 5: Behavioral analysis of Cntnap2-/- mice.
From: Dynamics of social representation in the mouse prefrontal cortex
(a) Open field test. Left: Mean distance travelled during test for WT (black, n = 8) and Cntnap2-/- mice (teal, n = 8). Two-sided Mann-Whitney U test, U = 28, P = 0.721. Right: Mean duration in center of arena. Two-sided Mann-Whitney U test, U = 28, P = 0.721. (b) Baseline sniffing quantifications for WT (black, n = 7 recording sessions) and Cntnap2-/- mice (teal, n = 6 sessions). Left: baseline sniffing frequency. Mann-Whitney U test, U = 26, P = 0.52. Right: baseline sniff amplitude. Mann-Whitney U test, U = 4, P = 0.014. Circles represents individual recording sessions. (c) Mean duration to find a buried food pellet for WT (black, n = 8) and Cntnap2-/- mice (teal, n = 8). Two-sided Mann-Whitney U test, U = 20, P = 0.234. (d) Mean duration of odor exploration, calculated as time sniffing odor port for WT (left, n = 7) and Cntnap2-/- mice (right, n = 7 mice). Duration is presented for first and second day of experiment (left to right) for each odor and each mouse. Two-way RM ANOVA with Bonferroni corrections. For WT: Fstimulus(5,30) = 15.444, P = 1.6 × 10−7; Fday(1,6) = 2.756, P = 0.148; Fstimulus*day(5,30) = 1.777, P = 0.148; for Cntnap2-/-: Fstimulus(5,30) = 11.862, P = 2.2 × 10−8; Fday(1,6) = 0.593, P = 0.470; Fstimulus*day(5,30) = 1.777, P = 0.203; (e) Left: Average latency to odor-evoked orientation responses for WT (black, n = 5) and Cntnap2-/- (teal, n = 6) mice in odor infusion chamber. Circles represent individual mice. Mixed-design RM ANOVA. Fgenotype(1,9) = 0.959, P = 0.352; Fstimulus(5,45) = 2.449, P = 0.048 with Dunnett’s test against clean air; Fgenotype*stimulus(5,45) = 0.163, P = 0.974. Right: mean probability of odor-evoked orientation responses. Mixed-design RM ANOVA. Fgenotype(1,9) = 0.040, P = 0.844; Fstimulus(5,45) = 3.304, P = 0.013 with Dunnett’s test against clean air; Fgenotype*stimulus(5,45) = 0.115, P = 0.988 (f) Locomotion levels of WT (black, n = 5) and Cntnap2-/- mice (teal, n = 6) in the odor infusion chamber during baseline and stimulus presentation (averaged across all presented odors). Two-sided Mann-Whitney U Test, For baseline: U = 7, P = 0.171; For stimulus: U = 10, P = 0.411. Circles represent individual mice unless otherwise indicated. For all panels: Mean ± s.e.m is presented. Note that some individual data points and error bars are covered by the mark of the mean. *P < 0.05. For detailed statistics information see Supplementary Table 2. M, male; F, female; B, banana; P, peanut butter; H, hexanal; CA, clean air. Base., baseline; Stim., Stimulus.
