Extended Data Fig. 10: Metabolic analysis of developmental glutamatergic neurons cultured in activated microglia-conditioned media.

a, Analysis of oxidative phosphorylation (ATP Production) and glycolysis (Baseline Glycolysis and Glycolytic Reserve) in HC glutamatergic neurons using a Seahorse Analyzer. Data are presented as mean±SEM. Two-Level Hierarchical Linear Mixed Effect Model was used for analysis (n=4 independent differentiations from 2 lines, each line with 2 independent differentiations). b, Analysis of oxidative phosphorylation (ATP Production) and glycolysis (Baseline Glycolysis and Glycolytic Reserve) in SCZ glutamatergic neurons using a Seahorse Analyzer. Data are presented as mean±SEM. Two-Level Hierarchical Linear Mixed Effect Model after log transformation was used for analysis of ATP Production and Glycolytic Reserve and Two-Level Hierarchical Linear Mixed Effect Model was used for analysis of Baseline Glycolysis (n=4 independent differentiations from 2 lines, each line with 2 independent differentiations). c, Analysis of oxidative phosphorylation in HC glutamatergic neurons vs. SCZ glutamatergic neurons cultured with or without activated microglia HMC3-conditioned media. Data are presented as mean±SEM. One-Level Hierarchical Linear Mixed Effect Model was used for analysis (n=4 independent differentiations from 2 lines, each line with 2 independent differentiations).