Extended Data Fig. 5: Cross-sample alignment of excitatory neurons from Mathys et al. recapitulates selective vulnerability in a RORB-expressing subpopulation.
From: Molecular characterization of selectively vulnerable neurons in Alzheimer’s disease
a, tSNE projection of excitatory neurons from Mathys et al.14 in the alignment space, colored by subpopulation identity (top) or individual of origin (bottom). b, Heatmap and hierarchical clustering of subpopulations and subpopulation marker expression (top subpanel); “High” and “Low” relative expression reflect above- and below-average expression, respectively (see Methods). Relative abundance of subpopulations in in AD cases vs. controls, separated by sex (second and third subpanels); for each sex, statistical significance of differences in relative abundance between AD cases vs. controls (cases n = 12, controls n=12, where n is the number of individuals sampled) was determined by beta regression and adjusted for multiple comparisons (see Methods). Expression heatmap of neocortical layer-specific genes from Lake et al.12 (fourth subpanel). Expression of selectively vulnerable EC excitatory neuron subpopulation markers (bottom subpanel). c, Heatmap of Pearson correlation between the gene expression profiles of excitatory neuron subpopulations from the EC vs. those from the prefrontal cortex in Mathys et al.14.
